NEURON Tutorial #14

Goals: Test kinetic scheme created by Payne and compare the result with Sonia's Ih result

Specific Aims:

Insert hPayne
Simulate
Compare with Sonia's Ih result

Downloads:

Alpha.hoc - For temporal summation experiment (alpha EPSCs)
Chirp.hoc - For chirp experiment
ExperimentControl.hoc - Experiment contols
Fitting.hoc - Procedures for curve fitting
GraphVI.hoc - Voltage and current graphs
Main.hoc - Program start here
ParameterControl.hoc - Parameter controls
Pulse.hoc - For pulse experiment (membrane time connstant)
RunControl.hoc - Run controls
Step.hoc - For current step experiment (Rin, sag, rebound slope)
VoltageClamp.hoc - Voltage clamp experiment
14.zip - Contains all files.

Summary

	Ih in Sonia's model	Payne's kinetic model
gbar (S/cm²)	4e-5	5e-5
Time Constant (ms)	24.3	24.3
Rin (MΩ)	56.9	54.9
Rebound Slope (mV/mV)	-0.17	-0.21
Temporal Summation (%)	11.0	9.25
Depolarizing Resonance Frequency (Hz)	3.5	3.8
Hyperpolarizing Resonance Frequency (Hz)	4.5	4.7
Vhalf (mV)	-81.1	-81.4
k (mV)	7.90	7.42
Time for Chirp Simulation (sec)	28.55	29.54

Steps and Notes:

Start "Main.hoc".
Measure membrane time constant:embrane time constant:
Click "Pulse (1 ms)" button. Click "Initiate & Run" button. Adjust gbar_h to 5e-5 so the resting membrane potential is -65 mV. Click "Initiate & Run" button. Click "Fit Decay" button.	Voltage and Current Traces Time constant = 24.3 (ms)
Measure Rin and Rebound Slope:
Click "Current Step (500 ms)" button. Click "Run Step Family" button. Click "Measure Rin (LR)" button. Click "Measure Rebound Slope (LR)" button.	Voltage and Current Traces:	Rin = 54.9 (M Ohm) Rebound Slope = -0.21 (mV/mV)
Temporal Summation:
Click "Five Alpha EPSCs (20 Hz)" button. Click "Initiate & Run" button. Click"Measure Temporal Summation" button.	Temporal Summation = 9.25 (%)
Chirp Experiment:
Click "Chirp" button. Click "Initiate & Run" button. Click "Characterize Chirp Response" button. Click "Fit Impedance Amplitude" button. Click "Plot Voltage Lag"	Voltage and Current Traces:	Impedance Amplitude: Depolarizing Resonance Frequency = 3.8 (Hz) Hyperpolarizing Resonance Frequency = 4.7 (Hz) Voltage Lag:
Voltage Clamp Experiment:
Click "Voltage Clamp" button. Click "Run Activation Step Family" button. Click "Fit GV Curve" button.	Voltage and Current Traces:	GV Curve: Vhalf = -81.4 (mV) k = 7.42 (mV)
Click "Run Deactivation Step Family" button. Click "Plot Tau" button.	Voltage and Current Traces:	Tau: