NEURON Tutorial #10

Goals: Perform chirp experiment

Specific Aims:

• Generate chirp current input.
• Record time and voltage during simulation.
• Analyze the impedance and the voltage delay.
• Export the data and plot it with Origin.

Steps:

• Start the environment by double-clicking Main.hoc.
• Click "Chirp".
• Click "Initiate & Run".
• Click "Characterize Chirp Response".

Downloads:

• Main.hoc - Program start here
• ExperimentControl.hoc - Experiment contols
• ParameterControl.hoc - Parameter controls
• RunControl.hoc - Run controls
• Graph.hoc - A window contains a voltage graph and a current graph
• FittingWindow.hoc - For linear regression analysis
• 1_Pulse.hoc - For pulse experiment (membrane time connstant)
• 2_Step.hoc - For step experiment (Rin, sag, rebound slope)
• 3_Alpha.hoc - For alpha experiment ( alpha summation)
• 4_Chirp.hoc - For chirp experiment
• 10.zip - Comprises all files.

Membrane potential in response to chirp current injection. Control Depolarizing resonance frequency = 4.06 Hz Hyperpolarizing resonance frequency = 4.85 Hz	Membrane potential in response to chirp current injection. Without Ih Depolarizing resonance frequency = 0.71 Hz Hyperpolarizing resonance frequency = 1.22 Hz
Impedance Amplitude	Voltage Delay