NEURON Tutorial #8

Goals: Insert Ih

Specific aims:

• Insert In, create parameter controls
• Simulate whiout Ih (gbar_h = 0, e_pas = -70, v_init = -70)
• Simulate with Ih (gbar_h = 5e-5, e_pas = -70, v_init = -65)

Downloads:

• Main.hoc - Program starts here
• ExperimentControl.hoc - Experiment Controls
• Parameters.hoc - Parameter Controls
  • gbar_h
  • e_pas
• RunControl.hoc - Run Controls
• Graph.hoc - A window includes a voltage graph and a current graph
• 1_TimeConstant.hoc - Controls for measuring membrane time constnat
• 1_SingleExponentialFitting.hoc - A window for single exponential fitting
• 2_InputResistance.hoc - Protocol contains a hyperpolarizing step. Calculates Rin and sag.
• 3_TemporalSummation.hoc - Controls for measuring temporal summation

Membrane Time Constant	Input Resistance	Temporal Summation
Without Ih
tau = 30 ms	Rin = 95 MΩ Sag = 0 %	Temporal Summation = 44%
With Ih
tau = 24 ms	Rin = 45 MΩ Sag = 21 %	Temporal Summation = 8%